A sensitive and specific ELISA immunocapture assay for rapid quantitation of influenza A/H3N2 neuraminidase protein
Identifieur interne : 000277 ( France/Analysis ); précédent : 000276; suivant : 000278A sensitive and specific ELISA immunocapture assay for rapid quantitation of influenza A/H3N2 neuraminidase protein
Auteurs : L. Gerentes [France] ; N. Kessler [France] ; M. Aymard [France]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1998.
English descriptors
- Teeft :
- Accurate quantitation, Adsorption, Antibody response, Antigen detection, Antigenic, Antigenic reactivity, Blank controls, Blank reaction, Chicken erythrocytes, Current vaccines, Detector antibodies, Detector antibody, Elisa, Elisa immunocapture assay, Elsevier science, Enzymatic activity, European pharmacopoeia, Gerentes, Hemagglutinin, Immunocapture, Immunocapture checkerboard titration, Inhibition test, Inhibition tests, Johansson, Kilbourne, Linear part, Microtiter plates, Monoclonal antibodies, Monoclonal antibody, Neuraminidase, Optimal dilution, Optimal dilutiond, Page integration, Pasteur merieux connaught, Polyclonal, Polyclonal rabbit, Precise estimation, Protein quantitation, Prototype strains, Quantitation, Rabbit antiserum, Rapid diagnosis, Reference antigen, Reference curve, Room temperature, Sample dilutions, Saturation step, Standard curve, Subunit vaccines, Trivalent, Trivalent suspension, Trivalent vaccine doses, Trivalent vaccines, Tween ether treatment, Vaccine, Validation, Validation method, Viral, Viral samples, Virol, Virological, Virological methods, Virus, Virus hemagglutinin, Virus infection, Virus neuraminidase, Whole virus.
Abstract
Abstract: Both HA and NA proteins elicit antibodies which have been shown to be capable of altering the course of infection. Nevertheless, while influenza virus vaccine standardization involves hemagglutinin (HA) and neuraminidase (NA) in terms of antigenic characterization, only HA protein quantitation is undertaken. An immunocapture ELISA (EIA) is described for N2 NA quantitation, based on the use of a highly specific monoclonal antibody (MAb) for capturing NA and an anti-NA antiserum for antigen detection. The amounts of NA in samples were deduced from the standard curve established by using purified NA. The NA-EIA is specific and detects as a little as 7 ng/ml. The capture and detector antibodies directed against A/Beijing/32/92 NA were shown to react with H3N2 prototype strains used in current influenza vaccines, provided that an antigenically matched reference NA is used as standard.
Url:
DOI: 10.1016/S0166-0934(98)00056-1
Affiliations:
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ISTEX:FA9386160BBC83FC8E17C1A5ED26B79A7A0D5C01Le document en format XML
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Aymard</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>W.H.O., National Influenza Centre, Laboratoire de Virologie, 8, Avenue Rockefeller, 69373, Lyon Cedex 08</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">73</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="195">195</biblScope></imprint><idno type="ISSN">0166-0934</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accurate quantitation</term><term>Adsorption</term><term>Antibody response</term><term>Antigen detection</term><term>Antigenic</term><term>Antigenic reactivity</term><term>Blank controls</term><term>Blank reaction</term><term>Chicken erythrocytes</term><term>Current vaccines</term><term>Detector antibodies</term><term>Detector antibody</term><term>Elisa</term><term>Elisa immunocapture assay</term><term>Elsevier science</term><term>Enzymatic activity</term><term>European pharmacopoeia</term><term>Gerentes</term><term>Hemagglutinin</term><term>Immunocapture</term><term>Immunocapture checkerboard titration</term><term>Inhibition test</term><term>Inhibition tests</term><term>Johansson</term><term>Kilbourne</term><term>Linear part</term><term>Microtiter plates</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Neuraminidase</term><term>Optimal dilution</term><term>Optimal dilutiond</term><term>Page integration</term><term>Pasteur merieux connaught</term><term>Polyclonal</term><term>Polyclonal rabbit</term><term>Precise estimation</term><term>Protein quantitation</term><term>Prototype strains</term><term>Quantitation</term><term>Rabbit antiserum</term><term>Rapid diagnosis</term><term>Reference antigen</term><term>Reference curve</term><term>Room temperature</term><term>Sample dilutions</term><term>Saturation step</term><term>Standard curve</term><term>Subunit vaccines</term><term>Trivalent</term><term>Trivalent suspension</term><term>Trivalent vaccine doses</term><term>Trivalent vaccines</term><term>Tween ether treatment</term><term>Vaccine</term><term>Validation</term><term>Validation method</term><term>Viral</term><term>Viral samples</term><term>Virol</term><term>Virological</term><term>Virological methods</term><term>Virus</term><term>Virus hemagglutinin</term><term>Virus infection</term><term>Virus neuraminidase</term><term>Whole virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Both HA and NA proteins elicit antibodies which have been shown to be capable of altering the course of infection. Nevertheless, while influenza virus vaccine standardization involves hemagglutinin (HA) and neuraminidase (NA) in terms of antigenic characterization, only HA protein quantitation is undertaken. An immunocapture ELISA (EIA) is described for N2 NA quantitation, based on the use of a highly specific monoclonal antibody (MAb) for capturing NA and an anti-NA antiserum for antigen detection. The amounts of NA in samples were deduced from the standard curve established by using purified NA. The NA-EIA is specific and detects as a little as 7 ng/ml. The capture and detector antibodies directed against A/Beijing/32/92 NA were shown to react with H3N2 prototype strains used in current influenza vaccines, provided that an antigenically matched reference NA is used as standard.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Gerentes, L" sort="Gerentes, L" uniqKey="Gerentes L" first="L" last="Gerentes">L. Gerentes</name></region><name sortKey="Aymard, M" sort="Aymard, M" uniqKey="Aymard M" first="M" last="Aymard">M. Aymard</name><name sortKey="Kessler, N" sort="Kessler, N" uniqKey="Kessler N" first="N" last="Kessler">N. Kessler</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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